Exploring the microstructure, antibacterial, and antioxidant features of Boswellia serrate extract -loaded Alginate/Gelatin porous Scaffolds for medical application

Document Type : Research Article

Authors

1 Biochemistry faculty of science mansoura university

2 Prof of biochemistry at national research center

3 Professor of Biochemistry, Chemistry Department, Faculty of Science, Mansoura University

4 Polymers and Pigments Department National Research center

Abstract

This study developed a novel sponge-like hydrogel composed of sodium alginate and gelatin (ALG/GEL) for the incorporation of Boswellia serrata extract (BSE), a promising bioactive agent for tissue regeneration. The synthesized BSE-loaded ALG/GEL scaffolds using emulsion freeze drying method. The obtained emulsion samples were characterized by TEM and DLS. The BSE nanoparticles exhibited an average diameter ranging from 196 ± 52 nm to 368 ± 142 nm, dependent on the BSE content. The bioactivity of the scaffolds was confirmed through a DPPH radical scavenging assay. The pure BSE extract demonstrated significant dose-dependent antioxidant activity with an IC₅₀ value of 72 μg/mL, confirming its inherent potency, though it was lower than the ascorbic acid positive control (IC₅₀ = 17.9 μg/mL). Crucially, the BSE-loaded scaffolds retained this beneficial property in a loading-dependent manner. The 20%BSE-loaded scaffold (IC₅₀ ≈ 78 μg/mL) was significantly more potent than the 10% loaded variant (IC₅₀ ≈ 135 μg/mL), highlighting the efficacy of the loading process. the in vitro findings lead to the conclusion that the developed BSE-loaded ALG/GEL scaffolds are effective antimicrobial agents. These multifunctional properties make the developed scaffold a highly promising candidate for advanced tissue regeneration applications.

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Mansoura Journal of Chemistry
Volume 68, Issue 4
December 2025
Pages 32-37

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