Uricase (EC 1.7.3.3) is an enzyme involved in the purine breakdown pathway. The enzyme was isolated from fenugreek (Trigonella foenum-graecum) leaves and purified by 80% ammonium sulfate, DEAE-cellulose and Sephadex G-200. The final specific activity was 230.76 Umg-1 protein and the final yield of purification was 2.87 % with 133.38-fold. The optimal pH and the optimal temperature were 8.0 and 40°C. The enzyme was inactivated by the chelating agent as α-α-dipyridyl and the IC50 value of α-α-dipyridyl was 9.03 mM.
Mohamed, H. E., Youssef, M. M., El-Moogy, R. A., & El-Shora, H. M. (2023). Purification and kinetics of uricase from fenugreek. Mansoura Journal of Chemistry, 62(4), 56-61. doi: 10.21608/mjcc.2023.411562
MLA
Howida E. Mohamed; Magdy M. Youssef; Rehab A. El-Moogy; Hamed M. El-Shora. "Purification and kinetics of uricase from fenugreek", Mansoura Journal of Chemistry, 62, 4, 2023, 56-61. doi: 10.21608/mjcc.2023.411562
HARVARD
Mohamed, H. E., Youssef, M. M., El-Moogy, R. A., El-Shora, H. M. (2023). 'Purification and kinetics of uricase from fenugreek', Mansoura Journal of Chemistry, 62(4), pp. 56-61. doi: 10.21608/mjcc.2023.411562
VANCOUVER
Mohamed, H. E., Youssef, M. M., El-Moogy, R. A., El-Shora, H. M. Purification and kinetics of uricase from fenugreek. Mansoura Journal of Chemistry, 2023; 62(4): 56-61. doi: 10.21608/mjcc.2023.411562
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